With the defending European champions trailing 2-0, the Juventus star fired home a 73rd-minute penalty which wasn’t enough to stop his side losing the Group B clash.
Ronaldo’s great rival Lionel Messi has scored 672 goals in 828 career matches for Barcelona and Argentina, with the Portuguese star moving to 700 in 974 games.
Ronaldo scored his first goal almost exactly 17 years ago and became the sixth player in history to reach 700 goals.
The full-time whistle in Kiev sparked wild scenes of celebration as Ukraine’s sixth victory from seven games moved them onto 19 points and an automatic spot at the Euro 2020 finals.
Portugal, by contrast, are back on 11 points and just one ahead of Serbia – which means they must win their last two fixtures against Lithuania at home and away at Luxembourg to guarantee their place.
Serbia remain in the mix after an Aleksandar Mitrovic brace helped them to a hard-fought 2-1 win against Lithuania.
Elsewhere on Monday, Turkey’s Kaan Ayhan punished France for wasting chances with a late equaliser in a 1-1 draw in the Stade de France which keeps Group H open.
Fellow substitute Olivier Giroud thought he had rescued the hosts with a goal just four minutes after coming off the bench in the second half of their Euro 2020 qualifier.
However, Ayhan’s far-post header from a free-kick with nine minutes to go left the two teams level on 19 points, with Iceland closing to within four points after their win over Andorra.
Cristiano Ronaldo’s goals record
- Games: 974 (812 club)
- Goals: 700 (605 club)
- Assists: 219 (190 club)
- Hat-Tricks: 54 (46 club)
- Penalties: Ronaldo 112 (103 club)
- Games: 812
- Goals: 605
- League: 418
- Champions League: 127
- Other cups: 55
- Hat-Tricks: 46
- Penalties: 103
- Sporting Lisbon: 5 (31 games)
- Manchester United: 118 (292 games)
- Real Madrid: 450 (438 games)
- Juventus: 32 (51 games)
- Assists: 191
- Premier League titles: 3
- FA Cup: 1
- English Football League Cup: 2
- La Liga titles: 2
- Serie A titles: 2
- Copa del Rey: 2
- Spanish Supercup: 2
- Serie A title: 1
- Supercoppa Italiana: 1
- Champions League: 5
- UEFA Super Cup: 2
- FIFA Club World Cup: 4
- European Championship: 1 (2016)
- Nations League: 1 (2019)
- Ballon d’Or: 5 (2017, 2016, 2014, 2013, 2008)
- Golden Shoe: 4 (2007-08, 2010-11, 2013-14 shared with Luis Suarez, 2014-15 – 48)